Skip to main content

About LISTERINE® antiseptic

Three healthcare professional in lab coats huddled around a microscope, collaborating on an examination

3 Essential Oils

Learn how this unique fixed combination is designed to deeply penetrate plaque biofilm and kill the bacteria that can lead to gingivitis.

LISTERINE® Antiseptic cleans where brushing can’t.

With the help of its unique formulation10 that includes the 3 Essential Oils (eucalyptol, menthol, thymol) LISTERINE® Antiseptic penetrates plaque biofilm killing bacteria in virtually 100% of the mouth.2,10-12 Unique to LISTERINE® brand Antiseptic, this fixed combination of the 3 Essential Oils formula is not offered by any other oral health care brand. 

To help patients kill the bacteria that can lead to plaque and gingivitis, recommend a LISTERINE® Brand rinse with the 3 Essential Oils formula, like LISTERINE® Total Care, for a complete oral care routine.

30 Second Exposure

LISTERINE® Antiseptic 30-second exposure

For maximum efficacy, the dosing of LISTERINE® Antiseptic rinses is 20mL for 30 seconds, twice a day. Why is only 30 seconds of rinsing necessary? 

In just 30 seconds of a full strength rinse, the technology behind LISTERINE® Antiseptic damages the cell surface of bacteria, interferes with growth, and increases the amount of time it takes for the bacteria to regrow11, 12, 15-18 as well as yields greater bacterial kill, and greater plaque reduction.19

Plaque biofilm

Scanning electron microscope (SEM) of plaque biofilm treated with LISTERINE® Antiseptic

Ruptured bacterial call wall

Close-up of SEM of ruptured bacterial cell wall in plaque biofilm treated with LISTERINE® antiseptic

*Study design: in this lab model, saliva-derived biofilms were grown on saliva-coated glass slides biofilms were cultured for 65 hours prior to mouthrinse exposure. Biofilms were stained to enable quantification of the live/dead cells.

Gram +/-

LISTERINE® Antiseptic bacteria kill

LISTERINE® Antiseptic lyses, kills, and inhibits growth of both gram-positive and gram-negative bacteria. The mouthwash anti-microbial action13 destroys the bacterial cell surface,11 inhibits biofilm growth,11 and increases the time it takes for bacteria to grow back.10

Bacteria that LISTERINE® Antiseptic destroys in the oral cavity include:

Gram-positive bacteria (+) 

  • Actinomyces naeslundii

  • Actinomyces viscosus

  • Staphylococcus aureus

  • Streptococcus mutans

  • Streptococcus sanguinis

  • Lactobacillus acidophilus

  • Enterococcus faecalis*

  • Streptococcus pyogenes

  • Streptococcus gordonii

Gram-negative bacteria (-) 

  • Aggregatibacter actinomycetemcomitans

  • Porphyromonas gingivalis

  • Prevotella intermedia

  • Bacteroides loescheii

  • Eikenella corrodens

  • Fusobacterium nucleatum

  • Klebsiella pneumoniaue*

  • Leptotrichia buccalis

  • Pseudomonas aeruginosa*

Gram-neutral bacteria 

  • Candida albicans (yeast)22

*Tests were run at 1-minute time point.